Phage Display Screen for Peptides That Bind Bcl-2 Protein
نویسندگان
چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملIdentiˆcation by the Phage-display Technique of Peptides That Bind to H7 Flagellin of Escherichia coli
The four peptides interacting with H7 ‰agellin of Escherichia coli were selected from a phage display library. The library was selected four times, and the interacting phage peptides were competitively eluted with H7 ‰agellin. An enzyme-linked immunosorbent assay (ELISA) showed that these peptides were reactive with the H7 ‰agellin in a dose-dependent manner. Among them, a D1 phage clone showed...
متن کاملHigh-Throughput Sequencing Enhanced Phage Display Identifies Peptides That Bind Mycobacteria
Bacterial cell wall components have been previously used as infection biomarkers detectable by antibodies. However, it is possible that the surface of the Mycobacterium tuberculosis (M. tb), the causative agent of tuberculosis (TB), also possesses molecules which might be non-antigenic. This makes the probing of biomarkers on the surface of M. tb cell wall difficult using antibodies. Here we de...
متن کاملPhage display affords peptides that modulate beta-amyloid aggregation.
As the population ages, the need to develop methods to understand and intercept the processes responsible for protein aggregation diseases is becoming more urgent. The aggregation of the protein beta-amyloid (Abeta) has been implicated in Alzheimer's Disease (AD); however, whether the toxic species is a large, insoluble aggregate or some lower order form is not yet known. Agents that can modula...
متن کاملGenome-Wide Prediction and Validation of Peptides That Bind Human Prosurvival Bcl-2 Proteins
Programmed cell death is regulated by interactions between pro-apoptotic and prosurvival members of the Bcl-2 family. Pro-apoptotic family members contain a weakly conserved BH3 motif that can adopt an alpha-helical structure and bind to a groove on prosurvival partners Bcl-xL, Bcl-w, Bcl-2, Mcl-1 and Bfl-1. Peptides corresponding to roughly 13 reported BH3 motifs have been verified to bind in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2010
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057110385816